Journal: Obesity Science & Practice

Article Title: Beyond Body Mass Index: Accurate Metabolic Disease‐Risk Phenotyping With 3D Smartphone Application

doi: 10.1002/osp4.70025

Figure Lengend Snippet: VAT mass as measured with DXA versus WC (upper panel) and WC/HC (lower panel) estimated manually with a flexible tape (left) and with the 3D smartphone application (right). One male with a technically inadequate DXA scan was excluded from this analysis. Both regressions in the upper panel are p < 0.001. VAT versus tape WC/HC in the lower panel, females, p < 0.01; other correlations are p < 0.001.

Article Snippet: Participants, clothed in solid‐colored spandex or other comfortable tight‐form fitting clothing, swim cap, and women in sports bras were asked to stand erect facing the tripod‐mounted smartphone (iPhone 14 Pro, Apple Inc, Cupertino, CA) positioned 1.7 m away for a single head‐to‐toe scan.

Techniques:

Journal: Microbial Cell Factories

Article Title: Boosting toxic protein biosynthesis: transient in vivo inactivation of engineered bacterial alkaline phosphatase

doi: 10.1186/s12934-020-01424-y

Figure Lengend Snippet: Biosynthesis and isolation of the recombinant His6-BAP protein from E. coli . a Overproduction of the recombinant His6-BAP in induced E. coli TOP10[pBAD-BAP1]. Lane M, LMW-SDS Marker Kit (GE Healthcare, cat. no 17044601); lane K, crude extract from E. coli TOP10; lane 1, E. coli TOP10[pBAD_BAP1] before induction; lanes 2–5, E. coli TOP10[pBAD_BAP1] after induction: 2 h, 4 h, 6 h and 20 h. b His6-BAP isolation and purification. Lane M, protein marker (Thermo Fisher Scientific, cat. no 26610); lane 1, extract from the induced E. coli TOP10[pBAD_BAP1] cells after PEI and ammonium sulphate treatment; lane 2, the metal affinity column flow-through; lanes 3 and 4, column wash; lanes 5–11, elution of homogeneous His6-BAP. Panel c. Comparative electrophoresis of His6-BAP and Sigma BAP. lane 1, Sigma BAP; lane 2, His6-BAP; Lane M, protein marker (Thermo Fisher Scientific, cat. no. 26619)

Article Snippet: E. coli HB101 {F − mcr B mrr hsd S20(r B − m B − ) rec A13 leu B6 ara -14 pro A2 lac Y1 gal K2 xyl -5 mtl -1 rps L20(Sm R ) gln V44 λ − } (Thermo Fisher Scientific (MA, USA)/GIBCO BRL) was used for wt phoA gene source.

Techniques: Isolation, Recombinant, Marker, Purification, Affinity Column, Electrophoresis